Further studies on diagnosis and control of african horse sickness disease in Egypt

lmmunogenic killed oil emulsion and freeze dried vaccines against African horsesickness could be from Type 9” dog strain” viscerotropic virus grown in monkey kidney stable cell line cultues. The virus was inactivated with Binary ethylene imine GBEI) by thafter 84 hours with BEl in concentrations of 0.002M and after 48 hours in concentrations of 0.003 M and within 18 hours in concentrations of 0.004 M.In order to enhance the immunogenicity of the two types of vaccines, incomplete Freund’s was addedin case of il adjuvant vaccine or the inactivated virus was concentrated 50X in case of freeze dried vaccine.