Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
On-line solid phase extraction coupled to capillary LC-ESI-MS for determination of fluoxetine in human blood plasma
Faculty
Science
Year:
2009
Type of Publication:
Article
Pages:
295-299
Authors:
Saber, Amr L
DOI:
10.1016/j.talanta.2008.11.016
Journal:
TALANTA ELSEVIER SCIENCE BV
Volume:
78
Research Area:
Chemistry
ISSN
ISI:000263634700046
Keywords :
Capillary liquid chromatography, Column switching, Fluoxetin hydrochloride, Mass spectrometry
Abstract:
An instrumental setup including on-line solid phased extraction coupled to capillary liquid chromatography-electrospray ionization-mass spectrometry (SPE-capLC-ESI-MS) has been constructed to improve the sensitivity for quantification of fluoxetine hydrochloride in human plasma. Prior to injection, 0.5 mL of plasma spiked with metronidazole (internal standard) was mixed with ammonium formate buffer for effective chloroform liquid-liquid extraction. The method was validated in the range 5-60 ng mL(-1) fluoxetine, yielding a correlation coefficient of 0.999 (r(2)). The within-assay and between-assay precisions were between (8.5 and 11\%) and (6.6 and 7.5\%), respectively. The method was used to determine the amount of fluoxetine in a healthy male 14 h after an intake of one capsule of the antidepressant and anorectic Flutin (R), which contains 20 mg fluoxetine per each capsule. Fluoxetine was detected, and the concentration was calculated to 9.0 ng mL(-1) plasma. In the preliminary experiments, conventional LC-UV instrumentation was employed. However, it was found that employing a capillary column with an inner diameter of (0.3 mm I.D. x 50 mm, Zorbax C(18)) increased the sensitivity by a factor of similar to 100, when injecting the same mass of analyte. Incorporating an easily automated C18 reversed phase column switching system with SPE(1.0 mm I.D. x 5.0 mm, 5 mu m) made it possible to inject up to 100 mu L of solution, and the total analysis time was 5.5 min. Published by Elsevier B.V.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف