Differnt methods of attenuation corynebacterium pseudotubercu-losis strains for possible vaccine production

The growth of C. ovis strains was completelty inhibited et PH 4 and PH 12 but attenuated at PHAll C.ovis strains could tolerate sadium chloride .concentrations up to 40% (satuTatcd solutions) in nutrientb:!::’oth ’’-.’hi19 the srowth was cOlnpletely inhibited on 7:;;sodit..l.1TI chlo:!::’ic’.9 in nutrient agar , All injected Guineapi0s rri t.h all treated strains shorled lOOS T:1orbidi t y,Bovine strains Vlere dfQd within 2-5 dayso It c~n beconc l udec. thHt tr’![1.tment \’ri th sodi 1..L7”!1 chloride increasedthe viru1 ence of bov i ne st:!::’ains and P ~ ?10 le si one vrex:echarQct erizecl by :;Jl~od ucLng diffuse gelatinou.’3 oedenat 01.1.8srrellingoAll Coovis strains covJ.cl rrro’:,r in 80clitlLl taurochole.tcbroth from concentrations 0005.-;-0, J% and complet01ylYU1101 ec~ R o’-t,Jil aLSO lJJ.1e 0::’~~D..r..1S[;1could 2-,Ot ~l’O:.OuJ ~ so~iu:Jul::’ Oil • .ioz-a t:!1e.n 0 0 05~ on soll(:;’ .P-’::’~~~.o =’ :r-(;Qt: .e nt - -i tiltc.urocholate had a [loWP::’:’:LlU’. t ten u.rt i oneffect on C.ovis strains of bovine origin, moderate.