Studies on the haemagglutinins of microsporum gypseum

Jlicro·sperwit.· gypseum isolates recovered from Egyptian soU were examined tor their capability ofhaemagglutinating red cells of different animals and birds” All isolates were found to agglutinate chick~n, duck ,nd sheep red cells but there was distinct var-j.ation. in the .. titre of. haemagglutination Microsporum gypseum iSQlate lio ~ ”F’ 37 - 82 which·gave a high haemagglutination titre in the pre1 i mi nary studies)was selected for fUrther studies ~During the course of subculturing a Tarient wild emerged from the or1ginal~isolate which produced onlymicroconidia but not macroconid1a •Both the wild isolate ( numerous macroconid1a and. few microconidia ) and mutant form ( only micro’:’conidia ) were used thruoghout this study • Studies to standirdize the haemagglutination test reveal.ed that the optimum conditions for the testwere I O.!5 % chicken red blood cells , 2~O % fungal Buspension t pH ~7 and incubation’ at room temperature •Tile best age of the culture was ,- 4 weeks •