Studies of some physical and chemical factors affecting the survival of buffalo spermatozoa

During the last few years some efforts have been made to produce proven bulls. As part of the general strategyof our Institute, attention was given to utilize the available buffalo bulls to the maximum through optimizingthe use of the produced semen. In line with the se views the present study has been initiated to evaluate theeffect of light on buffalo spermatozoa and to ilu.cidate those related factors e.g.time of exposure, type of’diluent ~ wave length, the presence of’ aromatic amino acids and the effect of sperm cell concentration.Four apparently normal, virile and sexually mature buffalo bulls, six to eight years age, kept at theexperimental farrn of the Pyramid Research Institute for A.ni.mal Reproduction, were used in the present study :Ejaculates collected from two to three bulls on each occasion were pooled to allow the required semen volume for a single trial and to eleminattion.Soon after collection, semen was assessed for mass activity, individual motility, percentage of live spermatozoa and sperm concentration.