A Study on some factors influencing the growth of baby hamster kidney cells in suspension ,ubed in foot & mouth disease vaccine production in Egypt

22- Comparative growth of BHK21 clone 13 using Brescia and 6M medium in 0.5 litre pyrex bottle show variation in final bell count, duration of the lag phase and in the mean generation time. Studying the effect of varying concentrationent of bovine serum 0I nactivation of bovine serum affect the growth rate of .BHK2l clone 13 in suspension either in 6M or Breceia media. BHK21 clone 13 derived from semi-oontinuous culture grewn we with a marked variation in oell density, duration of the Lag phase and mean generation tiine than these cells obtained from the refrigGrowing of BHK21 clono 13 cells in suspension using a New Brunswielc fcrmentor show the possibility of keeping the 5 litres containvr in continuous production for two weeks (7 cycles of 48 hours duration per cycle).Significant