( The present investigation was done in the department of Physiology, Faculty of Veterinary Medicine, Zagazig Atomic Power Do~d:iCairo U A R frem July 1980 to December,’ 1982•1155 irrunature mioe were usec males, females and virigins (treated with .hormonThe present study was conduc t ed on ~!~:5 inunature mice were given intraperitonea1ly a permissible dose level of the radioactive calcium 45 ca (0.05 ml containing 0.11 millicuri). After one week, animals were grouped into three groups according to theirThe first five animals of each subdivision were intrape- r1toneally injected with 10 Ug prolactin solution, the second ~;a.nimals •• re received 59 Ug prolactin. the immunogenicity of the vaccine, aluminium hydroxide gel wasadded as an adjuvant.After inocculation of a single dose of the vaccine, CF .Ab,HA.Ab, and SN .Ab developed within 4 weeks in inocculated horse,and when the second booster dose was injected after 4 weeks, muchhigher anti-bodies were present in sera after eight weeks post firstinjection.2.The use of SIR -8514 insecticide in controlling C. pipiens, succeed-ed under laboratory conditions and it was effective against thedifferent larval instars and pupae of C .pipiens .(Lab. strain) in thedilution 10-5 as it caused 100% reduction in emerged adults, whilein controlling the pupal stage of ~.pipiens (wild strain) the dilult-ion 10-3 of the investigated insecticide was effective as it caused99% reduction in emerged adult. Moreover I the investigatedinsecti cide was found to affect the fecundity of tested females ofC .pipiens as well as the longivity of both males and females of theemerged adults previously treated in the pupal stage with differentdi lutions of the insecti cide.