Some studies on Sex chromosomes In Albino Rat

The present study is an attempt to test the hypothesis that Lmmuno se Leo t Lon of gametes can be used as a method foridentifioation of x and y spermatozoa. The possibility of using suah a method for oontrol of sex ratio in animalswould be of great eo or.orni.c a.l value.A simple teohnique vra sdeveloped for oolleo1;ion rat spermatozoa from the oaud2 epididymis. Another apparatuswas designed for separation of fraotions of rat spermatozoasupposed to be rioh in y spermatozoa.Rabbit anti rat sperm antibodies were prepared by sub-outaneous injection of female rabbits with washed rat epid-idymal spermsem.:I.sified in Freund’s c omp Le t e adj uvant. The speoifioity of suoh antisera for rat spermatozoa was proved by the positive fluoresoenoe of spermatozoa treated with the oonjugated antisera and by the slide agglutination test where head ttitre of the prepared sperm antisera was determined by the Qapillary method.The isolated fraotions - supposed to be rioh in y sperms - were used for the absorplion of speoifio H - Yantisera present in the ~::”’•8pared rat sperm anti bodies.The effeot of the remaining antisera on x or y spermatozoa