Chromatographic Analysis for Fatty Acids and Lipid-Soluble Bioactives of Derris indica Crude Seed Oil

A combination of column chromatography (CC), gas chromatography (GC), thin layer chromatography (TLC) and liquid chromatography (LC) techniques were performed to analyze lipid classes, fatty acids and fat-soluble bioactives of Derris indica crude seed oil. Hexane extract of Derris indica oilseeds was found to be 56\%. Level of neutral lipids in the crude seed oil was the highest, followed by glycolipids and phospholipids, respectively. Linoleic followed by alpha-linolenic, palmitic and oleic were the major fatty acids in the crude seed oil. The ratio of unsaturated fatty acids to saturated fatty acids was higher in neutral lipid classes than in the polar lipid fractions. The oil was characterized by a relatively high amount of phytosterols, wherein the sterol markers were beta-sitosterol, campesterol and stigmasterol. gamma-Tocopherol was the major tocopherol while the rest being alpha-tocopherol. In consideration of potential utilization, detailed knowledge on the composition of Derris indica oil is of major importance.