Studies on the Newcastle Disease Virus AG68V) in the Internal Organsof the Fowl, SO AS to be Used Lately as Vaccinal Strain

The development of Newcastle disease was investigated in the organs of experimentally infected fowl’ with the virulent strain AG68V of Newcastle disease virus which was isolated from an outbreak of Newcastle disease in a vaccinated flock of chickens. The the virus in the host organs and tissue were assayed during the cOUrse of infection. The methods employed for virus detection and essay during the pathogenesis of infectionwere compared with each others. The associated tests included: Assay of infective virus in tissue by inoculation of embryonated avian egg. Demonstration of virus particles in tissue by haemagglu-tination, haemolysis tests. Demonstration of virus antigen in the tissue by agar gel precipitation test, passi~ haemagglutination adsorptiontest •.