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Studies on Foot & Mouth Diseases Virus in Cultivated Cells
Faculty
Veterinary Medicine
Year:
1979
Type of Publication:
Theses
Pages:
117
Authors:
laiia esmail ali El-shahawy
BibID
10092361
Keywords :
Virus Diseases
Abstract:
•- Baby hamster kidney cells were grown in suspension culturesusing Eagle’s medium and the same medium with and/or without some non defined protein components for the purpose ofminimising the presence of those non-defined ptotein supplements and its influ2- The effect of varying concentrations of bovine serum with and without amino acid mixture supplement, tryptic soy broth and proteose peptone were investigated.3- from an average starting cell count of about 0.5 x 106 cells/ml, using Eagle’s medium with different percentages of bovine serathe results achieved showed that an average high cell density of 1.87 x 106 cells per ml. was obtained on the 4th passagewhen using complete Eagle’s medium supplemented with 10%bovine serum. Using the same batch of cells but with less ppercentage of bovine serum, the cell density did not exceed lxl06cells/ml.; which was confirmed by both pH changes andglucose utilization ra
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