Factors affecting clostridium tetani toxin

1) Mueller and miller medium had the essential components for t oxt.n production. Therefore cultivation of this medium with Cobenhagen strain (501 b) and incubation for 7 days at 35°C produced toxin of high potency (320.000 M.L.D.) in COillparison to the 2) The use of 1 % peptone solution or 0.2 % gelatin as a diluents for the toxin produced in Mueller and Miller medium were the best diluents as they verified any minute amount of the toxin in the filtrate by protecting it from denaturation and ac.sorbtion