Trial For The Use Of Inactivating Agents For Newcastle Disease Vaccine

- 69 - The present investigation deals with the preparationof Luc c t Lvat o d NDV vacc Lno s u s i.ng different inactivators Fad adjuvants in 3D attempt tc select the most potent vaccine that may replace the living vacbine prepared in eggsin order to con(SPF) E.;ggs in Egypt k. local VV1~DV stir ad n of virus i801ated in the co uc se of out br- aks of disease in 1974, was utilized in preparation of the vaccines. The infectivity titre of a allanto amniotic fluid reached 108•5 EID50•The harvest was concentratEd with polyethylen glycol (PEG) to give a titre of 109•5 EID50• ri variety of methods have been used forinactivation of concGl1trated NDV pre-pared in eggs including Eeta pcr-opd,o lactone (BPL) in a (0.2 % for 2 hours at 37°C), Etbyl Ethylene Imine (EEI)in a (0.05 % for 2 hours at 37°0). Formalin (1 % for 2hours at 37°0) and heat exposure at (58°C for Yz hour).The ad juvant s used for EFL inactivated vaccine in-cluded oil, aluminium hydroxide gel and sodium alginate a d juvant a, Tha procedure adopted for evalueting the immune response included adopted for evaluating the immune response included the haemagglutination inhibtion (HI), I.M. chollenge test using 105