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Succeptility of Different Cell Cultures to Foot-and- Mouth Disease Virus
Faculty
Veterinary Medicine
Year:
1979
Type of Publication:
Theses
Pages:
86
Authors:
Adel Omar Abdel Rahnan
BibID
10092448
Keywords :
Veterinary Microbiology
Abstract:
1. Comparative studies on the cultivation of four types of kidney cell cultures obtained from different species of animals were investigated. The primary cell cultures variQdin the time of appearance and developing of confluent monolayer sheet. The most rapid, once were those obtained from kidneys of Bovine Embryo (BEK) and Buffalo Embryo (BfEK),while Camel Embryo kidney (CEK) and sheep kidney (ShK) need for development of monolayer sheets one and two days respectively more than that needed by the other two types.2. The cytopathogenicity of FHDV, strain °1/2/72 - Egypt on the studied cultures, wainoculation and the time decreased by successive passagingo 3. The multiplication of FI’1DV strain 2 virus yield of different titers. The lowest infective titer (103•75 TClD50/ml.) was obtained with CEX cell cultures. Successive passages of FMDV on other an increc:,se in both its infectivity titer and complement fixing activity. L}. The maximum FMDV yield fran whole infected culture was obtained as rapidly as 21-24 hous post - inoculation regardless the type of trypsinized cell cultures infected, with thevity support the infectivity findings.
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