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L-glutaminase production by Trichoderma koningii under solid-state fermentation
Faculty
Science
Year:
2009
Type of Publication:
Article
Pages:
243-250
Authors:
El-Sayed, Ashraf S. A
DOI:
10.1007/s12088-009-0020-2
Journal:
INDIAN JOURNAL OF MICROBIOLOGY SPRINGER
Volume:
49
Research Area:
Biotechnology \& Applied Microbiology; Microbiology
ISSN
ISI:000269927400009
Keywords :
L-glutaminase, Filamentous fungi, Trichoderma koningii, Solid state fermentation
Abstract:
Solid state fermentation was conducted for the production of L-glutaminase by Trichoderma koningii Oud.aggr. using different agro-industrial byproducts inlcuding wheat bran, groundnut residues, rice hulls, soya bean meal, corn steep, sesamum oil cake, cotton seed residues and lentil industrial residues as solid substrates. Wheat bran was the best substrate for induction of L-glutaminase (12.1 U/mg protein) by T. koningii. The maximum productivity (23.2 U/mg protein) and yield (45.0 U/gds) of L-glutaminase by T. koningii occurred using wheat bran of 70\% initial moisture content, initial pH 7.0, supplemented with D-glucose (1.0\%) and L-glutamine (2.0\% w/v), inoculated with 3 ml of 6 day old fungal culture and incubated at 30A degrees C for 7 days. After optimization, the productivity of L-glutaminase by the solid cultures of T. koningii was increased by 2.2 fold regarding to the submerged culture.
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