Studies on field applications of frozen bull and buffalo bull Semen

The .wide application of A. 1. program using frozen semen technique inEgypt, necessitate the reduction of the heavy load that should be carricdoutduring insemination (L. N. Dewars and Refrigerators) tor long time. A situationthat trigger the need for studying the condition that takes place in the thermalenvironment of the thawing bath when more than one straws are thawed andincubated for up to 3 hr. in the the11110s flask. In the present study frozen semenstraws were used from two cattle bulls and two buffalo bull. While bull frozensemen straws are manufactured in Beni-Suif frozen semen unit using Tris diluent,buffalo frozen semen straws were prepared in Abbasia Frozen Semen Unit byusing skimmilk diluent.Frozen semen straws 11’0m each cattle and buffalo bulls were exposed tothree thawing temperature 35 C, 38 C and 40 C . At each temperature threegroups of straws 3. 6 or 12 are thawed ill non-thermostatically controlled.thermos-flasks. A sample of 3 straws are picked lip a Iter 0, 1, 2, and 3 hours andexamined for percentage of post-thaw motility, percentage of bent tail andpercentage of intact acrosome, also the DROP of the temperature was determinedafter the a fore mentioned holding times.A second laboratory experiment was designed aimed at recognizing theeffects of the semen DROP site in the straw on the percentage of spermatozoalmotility 50 straws from each bull were thawed in a water bath at 35 C for 30 sec,After thawing three DROPs were taken frorn each straw on one slide the firstclose to the laboratory seal (site A) , the second from the middle line ofthe straw (site 13) and the third close to the factory cotton seal (site C).