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Induction of Pyridine Alkaloid Formation in Transformed Root Cultures of Nicotiana tabacum
Faculty
Not Specified
Year:
2009
Type of Publication:
Article
Pages:
869-874
Authors:
Wink, Michael, Zayed, Rawia
Journal:
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH
Volume:
64
Research Area:
Biochemistry \& Molecular Biology; Pharmacology \& Pharmacy
ISSN
ISI:000274113500019
Keywords :
Nicotiana tabacum, Pyridine Alkaloids, Hairy Root Culture
Abstract:
Hairy root cultures of Nicotiana tabacum. were set up by excised root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analyzing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of pyridine alkaloids, mainly nicotine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid, in order to stimulate the biosynthesis of pyridine alkaloids. Profile and amounts of pyridine alkaloids were analyzed using capillary GLC-MS. Treatment of the cultures with methyljasmonate (50 mu M) increased the alkaloid accumulation ca. 7-fold up to a level of 0.58 mg/g fresh weight as compared to untreated controls. Quercetin (200 mu M) enhanced the alkaloid production ca. 4-fold (0.34 mg/g fresh weight) within 24 h. In contrast salicylic acid in all tested concentrations decreased the alkaloid level to 1 mu g/g fresh weight. Also the inhibitory effect of salicylic acid on the elicitation effect of methyljasmonate and quercetin was investigated.
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