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Spectral and Spatial Characterization of Protein Loaded PLGA Nanoparticles
Faculty
Pharmacy
Year:
2010
Type of Publication:
Article
Pages:
1180-1192
Authors:
Zidan, Ahmed S, Habib, Muhammad J, Khan, Mansoor A, Rahman, Ziyaur
DOI:
10.1002/jps.21928
Journal:
JOURNAL OF PHARMACEUTICAL SCIENCES JOHN WILEY \& SONS INC
Volume:
99
Research Area:
Pharmacology \& Pharmacy; Chemistry
ISSN
ISI:000275215100007
Keywords :
nanoparticles, encapsulation, biodegradable polymers, near infrared spectroscopy, partial least squares, and cyclosporine A
Abstract:
The objective of this study was to evaluate near infrared (NIR) spectroscopy and imaging as approaches to assess drug contents in poly(dl-lactide-co-glycolide) (PLGA) based nanoparticles of a model protein, cyclosporine A (CyA). A 6-factors 12-runs designed set of experiments with Plackett-Burman (PB) screening was applied in order to examine the effects of drug loading (X(1)), polymer loading (X(2)), emulsifier concentration (X(3)), stirring rate (X(4)), type of organic solvent (X(5)), and ratio of organic to aqueous phases' volumes (X(6)), on drug entrapment efficiency (EFF). After omitting the factors with nonsignificant influences on EFF, a reduced mathematical relationship, EFF = 48.34 + 7.3X(1) - 29.95X(3), was obtained to explain the effect of the significant factors on EFF. Using two different sets for calibration and validation, the developed NIR calibration model was able to assess CyA contents within the 12 PB formulations. NIR spectral imaging was capable of clearly distinguishing the 12 formulations, both qualitatively and quantitatively. A good correlation with a coefficient of 0.9727 was obtained for constructing a quantile-quantile plot for the actual drug loading percentage and the \% standard deviation obtained for the drug loading prediction using the hyperspectral images. (C) 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99:1180-1192, 2010
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