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Screening of strains of lactococci isolated from Egyptian dairy products for their proteolytic activity
Faculty
Agriculture
Year:
2010
Type of Publication:
Article
Pages:
758-764
Authors:
Sitohy, Mahmoud, Chobert, Jean-Marc, Haertle, Thomas, El-Ghaish, Shady, Choiset, Yvan, Dalgalarrondo, Michele, Ivanova, Iskra
DOI:
10.1016/j.foodchem.2009.11.007
Journal:
FOOD CHEMISTRY ELSEVIER SCI LTD
Volume:
120
Research Area:
Chemistry; Food Science \& Technology; Nutrition \& Dietetics
ISSN
ISI:000275014200018
Keywords :
LAB, Lactococcus, Cheese, Proteolysis
Abstract:
A collection of cocci isolates (293) obtained from traditional Egyptian dairy products collected from four Egyptian regions yielded 151 lactic acid bacteria (LAB) cocci isolates. Among them, 24 isolates were characterised as highly proteolytic. SIDS-PAGE showed that 6 isolates were the most proteolytically active, which were classified into Enterococcus faecalis HH22 (4 isolates) and Enterococcus faecium DO623 (2 isolates). The proteolytic activity of E. faecalis was higher than that of E. faecium (particularly on beta-casein). The maximal degradation of milk proteins was achieved at pH 6.5-7.2 (E. faecalis) or pH 6.5 (E. faecium) and at 42 degrees C for both strains. The proteolytic activities of the two strains were inhibited mostly by the presence of EDTA, showing that their proteases belong mainly to metalloproteases. A slight inhibition of proteolysis by PMSF in the case of E. faecalis HH22 suggests a limited inclusion of serine proteases in its protease system. (C) 2009 Elsevier Ltd. All rights reserved.
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