Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Standardized Single-Platform Assay for Human Monocyte Subpopulations: Lower CD14(+)CD16(++) Monocytes in Females
Faculty
Medicine
Year:
2010
Type of Publication:
Article
Pages:
823-830
Authors:
Marei, Ayman, Boghdadi, Ghada, Heimbeck, Irene, Hofer, Thomas P. J, Eder, Christiane, Wright, Adam K, Frankenberger, Marion, Scherberich, Juergen, Zieger-Heitbrock, Loems
DOI:
10.1002/cyto.a.20942
Journal:
CYTOMETRY PART A WILEY-BLACKWELL
Volume:
77A
Research Area:
Biochemistry \& Molecular Biology; Cell Biology
ISSN
ISI:000281508100005
Keywords :
monocyte heterogeneity, gender, exercise, glucocorticoids
Abstract:
We present a novel single-platform assay for determination of the absolute number of human blood monocyte subpopulations, i.e., the CD14(++)CD16(-) and the CD14(+)CD16(++) monocytes. A four-color combination of antibodies to CD14, CD16, CD45, and HLA-DR reduces the spill-over of natural killer cells and of granulocytes into the CD14(+)CD16(++) monocyte gate. For these CD14(+)CD16(++) monocytes, the intra-assay coefficient of variation (CV) was 4.1\% and the inter-assay CV was 8.5\%. Looking at a cohort of 40 donor S aged 18-60 years, we found no age dependence. There was however an effect of gender in that females had lower CD14(+)CD16(++) monocytes (45.4 +/- 13.5 cells/mu l) compared with males (59.1 +/- 20.3 cells/mu l) (P < 0.02). Using this novel approach, we can confirm that exercise will lead to more than three-fold increase of the CD14(+)CD16(++) monocytes. Also, we show that therapy with low doses of glucocorticoids will deplete these cells. This robust single-platform assay may be a useful tool for monitoring the absolute number of monocyte subpopulations in health and disease. (C) 2010 International Society for Advancement of Cytometry
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف