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Molecular evaluation of a new highly sensitive aryl hydrocarbon receptor in ostriches
Faculty
Veterinary Medicine
Year:
2013
Type of Publication:
Article
Pages:
1921-1929
Authors:
Fujisawa, N, Darwish, W. S, Ikenaka, Y, Kim, E, Lee, J, Iwata, H, Nakayama, S, Ishizuka, M
DOI:
10.3382/ps.2013-03090
Journal:
POULTRY SCIENCE POULTRY SCIENCE ASSOC INC
Volume:
92
Research Area:
Agriculture
ISSN
ISI:000320443400027
Keywords :
ostrich, avian species, aryl hydrocarbon receptor, CYP1A, dioxin
Abstract:
A 1,000-fold difference has been reported in dioxin sensitivity between avian species. This difference is because the 2 amino acids in the type 1 aryl hydrocarbon receptor (AhR1), at positions 325 and 381, correspond to Leu324 and Ser380 in chickens. The chicken had been reported to be the only avian species to possess a sensitive form of AhR1. This is the first study to reveal that the ostrich (Struthio camelus), a nonchicken species, also has a pair of amino acids (Ile-325 and Ser-381) that show high ligand affinity. However, the alignment of the AhR1 cDNA sequence showed that the AhR sequence in the ostrich was different than that of other avian species even though the critical amino acids were observed at positions 325 and 381. Ostrich AhR1 was also evaluated in a heterologous expression study. Ostrich AhR1 showed very high transcriptional activity of the cytochrome P450 1A5 (CYP1A5) gene in African Green Monkey Cercopithecus aethiops kidney cells (COS-7) treated with Sudan III. In primary cultures of ostrich kidney cells, CYP1A5 expression was induced by Sudan III at a lower (or almost identical) concentration to that observed in the chicken. The present study revealed a new AhR ligand sensitive avian species (i.e., the ostrich).
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