A Biochemical Study on Hepatic Acute Phase Proteins in Certain Metabolic Disorder

Present work has been focused mainly on :1- Inflammatory markers in response to diabetic induction using STZ rats. Correlation between these markers and other metabolic parameters endothelial dysfunction, oxidative stress and lipogram pattern.3-Potential role of anti-inflammatory, hypolipidemic, hypoglycemic and natural antioxidant agents either individually or in combination form in STZ diabetic rats . To fulfil these requirements synthetic anti-diabetic (glimepiride), natural antioxidant (catechin), hypolipidemic agents (Pravastatin), anti-inflammatory (aspirin ) were used .Certain biochemical parameters were selected for evaluation. These are namely serum glucose, CRP, SAA, TNF-α , IL-6 , NO , susceptibility of non HDL-C to oxidation and lipogram profile .Representative samples of rat aorta (2 portions) were removed and processed instantly for determination of aortic superoxide anion production in addition to histopathological examination . This is to configurate cellular changes and to correlate it with other biochemical parameters.Experimental animals: Rats were classified randomizely into 2 main experiments as follows:Experiment I:1- Normal control group (n = 12)2- STZ diabetic rats “diabetic control group” (n = 15)3- STZ rats received glimepiride (n = 15)4- STZ rats received catechin (n = 15)5- STZ rats received pravastatin (n = 15)6- STZ rats received aspirin (n = 15)Experiment II:In this experiment the animals were treated with a combined form of the drugs used