| Abstract: |
The current study aimed to reduce the urinary catheter contamination by biofilm forming Gram negative bacteria, e.g. E. coli, Klebsiellapneumoniae, Proteus mirabilis and Pseudomonas aeruginosa and Grampositive bacteria, e.g. Staphylococcus epidermidis and staphylococcusaureus. Also the study aimed to detect the genes responsible for biofilmformation in different bacteria and suggestion of different coating systemsfor the urinary catheters to reduce catheter associated urinary tractinfections (CAUTI).Among the 128 urinary catheters collected, one hundred and eight(108) were showed adherent bacteria. from these urinary catheters, 150isolates were obtained and identified as: E. coli 40 isolates (26.7%), 28isolates (18.7%) as Klebsiella were identified, 20 (13.3%) Proteusisolates, 22 (14.7%) Pseudomonas isolates, S. epidermidis isolates 28(18.7%) and 12 (8%) isolates S. aureus (Table 6 and Figure 9).Identification of clinical isolates were based mainly on themorphological, culture characteristics and the biochemical tests accordingto Koneman et al. (1997) and Forbes et al. ( 2007). Biofilm productionis an important pathogenic factor which facilitates the adherence ofmicroorganism to medical devices and protects them from the hostimmune system and antimicrobial therapy.As shown in (table 7 and figure 10), the clinical isolates wereclassified according to the degree of biofilm formation and adherence into4 groups which are non, weak, moderate and high producers.In this study, As (table 7 and figure 10) revealed, Gram negativebacteria were more predominant than Gram positive bacteria as highlybiofilm producers. Where, 36.4% of Pseudomonas isolates were highlyproducers followed by E. coli and Proteus isolates (30% each). Klebsiella125isolates were the least highly biofilm forming bacteria among Gramnegative isolates.
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