SOME OPTIMAL CULTURE CONDITIONS FOR PRODUCTION OF CYCLOSPORIN A BY FUSARIUM ROSEUM

A suitable chemically defined culture medium was selected and some optimal conditions for the production of the highly immunosuppressive compound, cyclosporin A (Cyc A) are reported. Medium of the following composition was favorable for the production of Cyc A by Fusarium roseum: glucose, 30; NaNO(3), 2; KH(2)PO(4), 1; MgSO(4)center dot 7H(2)O, 0.5 and KCL, 0.5 (g/l). Maximum productivity of Cyc A was achieved at pH 6.0 when 50 ml of the fermentation medium/250 ml flask, inoculated with five fungal agar discs (6 mm, diameter) of 7-days old F. roseum culture after incubation at 30 degrees C at 120 rpm for 7 days.