Pharmaceutical Studies of interations Between Certain Anti-Inflammatory Drugs And plasma Components Or Their Substitutes

AbstractUpon entry into the blood circulation, most drugs bind to plasma proteins and/or other blood components (e.g. red blood cells). It is generally accepted that only the free drug molecules can arrive at the site of action that will be enzymes or receptors, and at steady state drug effect are directly proportional to the free concentration in plasma. Free drugs are rapidly distributed into tissues, resulting in direct binding to tissue proteins. Therefore, the binding of drugs to plasma and tissue proteins is common and is considered to be an important factor determining the pharmacokinetics and pharmacodynamics of drugs. In most cases, the binding of drugs to plasma and tissue proteins is reversible and there is equilibrium between bound and unbound drugs. However, in less common instances, drugs may covalently bind to plasma and tissue proteins, leading to the formation of drug–protein adduct. The binding of drugs to human serum albumin and also different factors that affect binding should be examined.Piroxicam and Ketoprofen are two anti-inflammatory drugs widely used in our country. The work in this thesis deals with the binding of these drugs to bovine serum albumin as well as factors that affect the binding process. The release of these drugs from drug- protein complex was also studied.