Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Spectrophotometric determination of diphenhydramine hydrochloride in pharmaceutical preparations and biological fluids via ion-pair formation
Faculty
Science
Year:
2010
Type of Publication:
Article
Pages:
265-270
Authors:
El-Didamony, Akram M, Moustafa, Moftah A
DOI:
10.1016/j.arabjc.2010.06.014
Journal:
ARABIAN JOURNAL OF CHEMISTRY KING SAUD UNIV PRESS, KING SAUD UNIV
Volume:
3
Research Area:
Chemistry
ISSN
ISI:000290141800011
Keywords :
Diphenhydramine hydrochloride, Patent blue, Methyl orange, Bromocresol purple, Eriochrome black T, Tablets, Urine, Spectrophotometry
Abstract:
A simple, sensitive and accurate spectrophotometric method has been described for the assay of diphenhydramine hydrochloride (DPH) in raw material and in biological samples. The method is based on extraction of DPH into dichloromethane as ion-pair complexes with patent blue (PB), eriochrome black T (EBT), methyl orange (MO) and bromocresol purple (BCP) in acidic medium. The coloured species exhibited absorption maxima at 632, 514, 428 and 414 rim for PB, EBT, MO and BCP, with molar absorptivity values of 1.32 x 10(5), 2.36 x 10(4), 3.68 x 10(4) and 3.07 x 10(4) l mol(-1) cm(-1) respectively. The reaction conditions were optimized to obtain the maximum colour intensity. Beer's law was obeyed with a good correlation coefficient (0.9982-0.9993) in the concentration ranges 0.5-3, 2.0-16, 2.0-10 and 1.0-10 mu g ml(-1) for PB, EBT, MO and BCP methods, respectively. The composition ratio of the ion-association complexes was found to be 1:1 in all cases as established by Job's method. The conditional stability constant (K(f)) and the free energy changes (Delta G degrees) were determined for all complexes formed. The proposed method was successfully applied for the determination of DPH in tablets and human urine with good accuracy and precision. Statistical comparison of the results with those obtained by the official method showed good agreement and indicated no significant difference in accuracy and precision. (C) 2010 King Saud University. All rights reserved.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف