Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry
Faculty
Pharmacy
Year:
2010
Type of Publication:
Article
Pages:
3303-3316
Authors:
Ismaiel, Omnia A, Karnes, H. Thomas, Jenkins, Rand G, Zhang, Tianyi
DOI:
10.1016/j.jchromb.2010.10.012
Journal:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ELSEVIER SCIENCE BV
Volume:
878
Research Area:
Biochemistry \& Molecular Biology; Chemistry
ISSN
ISI:000285118100015
Keywords :
Matrix effect, Lipids, Quantitative analysis, Selectivity
Abstract:
Matrix effects caused by compounds endogenous to the biological sample are a primary challenge in quantitative LC/MS/MS bioanalysis Many approaches have been developed to minimize matrix effects such as optimization of sample extraction procedures and use of isotopically labeled internal standards Unexpected matrix components may still remain undetected however because of the selective mass transitions monitored during MS/MS analysis Glycerophosphocholines are the major phospholipids in plasma that have been widely shown to cause significant matrix effects on electrospray ionization efficiencies for target analytes The purpose of this work was to investigate potential matrix effects resulting from different endogenous lipid classes including phospholipids acylglycerols and cholesterols in order to establish a library for the relative presence of these components in biological sample extracts obtained by commonly used sample preparation techniques Thirteen compounds were selected which were representatives of eight phospholipids classes mono di tnacylglycerols cholesterol and cholesterol esters Post-column infusion experiments were carried out to compare relative ion suppression effects of these compounds Chlorpheniramine and loratadine were selected as model test analytes A Concentration Normalized Suppression Factor (\%CNSF) was defined to allow comparison of ion suppression effects resulting from different endogenous lipids according to their typical concentrations in human plasma and erythrocytes A simple LC/MS/MS method was developed to monitor these endogenous components in sample extracts and their extraction recoveries from a plasma pool were compared using protein precipitation liquid-liquid extraction supported-liquid extraction solid phase extraction and Hybrid SPE-precipitation methods Endogenous lipid components other than GPChos such as cholesterols and tnacylglycerols may result in significant matrix effects and should be monitored during method development No single extraction procedure was efficient in removing all of the various lipid components Use of the results presented here along with a consideration of analyte chemical structure the type of matrix and the type of sample preparation procedure may help a bioanalytical scientist to better anticipate and minimize matrix effects in developing LC/MS/MS-based methods (c) 2010 Elsevier B V All rights reserved
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف