Invitro Studies on Escherichia Coli Strains Isolated from Urinary Tract Infections In Different Patient Groups

Urinary tract infection is the commonest disease of urinary tract and several rapid screening procedures have been recommended for estimation of urine specimens to decide whether or not a sample contains of a gram-stained smear of fresh, unceatrifuged urine and the catalase activity of fresh urine.For isolation of E.coli , direct plating of the specimen on selective and differential media done e.g., MacConkey agar , E.M.B. agar , bromocresol purple-lactose agar,.. Several of more recently developmed and commercially available biochemical tests for Enterobacteriaceae ( E. coli ) were evaluated that are required to identify all potential pathogens rapidly and accurately . A simplified biochemical routine was developed for thus purpose consisting of: triple sugar iron agar; urea agar; indole ; Methyl red ; Voges proskauer Phenylalanine ; Motility ; Pot-cyanide ; Moeller’s 4-tube decarboxylases and citrate utilization test. Susceptibility testing is routinely performed usually by either the disc diffusion technique or a dilution method.