| Abstract: |
Wedelia prostrata F-look ci Am. (Hemsl.) belongs to Family Compositae. Literature survey showed that genus Wedelia s characterized by the presence of tetracyclic diterpenes (Kaurenes.1 and sesquiterpene lactones (eudesmanolides), nothing could be traced about the biological activity and the chemical constituents of W. prostrata, this stimulated the present work which includes the following: <Chapter 1: Isolation and identification of the main constituents from the hexane soluble fraction of W. prostrata.Chapter [1: [solation and identification of the main constituents from the chloroform soluble fraction of W prostrata.Chapter Iii: Iofation and identification of the main constituents from the ethyl acetate soluble fraction of W. prostrata.Chapter IV: Screening of the MeOl I extract of W prostrata for the inhibitory effect on human immunodeuiciencv virus type Iprotease.Chapter IIsolation and identification of the main constituents fromthe hexane soluble fraction of IV prO5’tratIl.1— Extractioii and fractionation of the plant:The air dried powdered herb (3.8 Kg) was extracted by maceration in MeOH at room temperature till exhaustion. [‘he N4e0[I extract was diluted with water and subjected to fractionation by hexane, chloroform, ethyl acetate and butanol.2- Isolation:About 15 gm of the hexane fracnori were fractionated by SiO CC using Flex-EtOAc mixtures. Each fraction was subjected to co)umn ch.romatorgraphy using RP- 18, the elution was achieved by either \leCHor CH3-F1O mires The rnixture of compounds 5 and 6 wassolated by E-[PLC using ODS and v1eOH-i[O 95:5). Compounds 1-10 were obtained.3- Identification of the isolated compounds:The isolated compounds have been charactenzed on the basis of spectroscopic data (IJV, ER, 1ff t3C-NvEP F{H-COSY, CE4-COSY, DEPT ‘3CN4ER, Difference NCE, COLC)C or HMBC and FAB-MS or E[-vtS) and listed in Table (44).Chapter IIIsolation and identification of the main constituents from thechloroform soluble fraction of W. prostrata.1- Isolation:About 30 gm of the chioroform fraction were fractionated by SiO CC using CRCI]-EtOAc mixtures folloed by CHC13-v1eOH mixtures. Each fraction was recltromacographed on SO CC and the purification of compounds II, 12 and 13 was achieved by PTLC. Tfr mixtures of compounds 14,15 and 16,17 were separated by using F-IPLC (ODScolumn, MeO[-{-H,O (8:2)).2- Identification of the isolated compounds:The isolated compounds were identified according to theu spectraldata (UV, [R 1H-, UCNMR, KH-CQSY, CH-COSY, DEPT UCNMR, Difference NOE, COLOC or EI?vfRC and FAB-vIS or EI-MS).
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