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Isolation of a New Trypsin Inhibitor from the Faba Bean (Vicia faba cv. Giza 843) with Potential Medicinal Applications
Faculty
Agriculture
Year:
2011
Type of Publication:
Article
Pages:
64-72
Authors:
Fang, Evandro Fei, Hassanien, Abdallah Abd Elazeem, Wong, Jack Ho, Bah, Clara Shui Fern, Soliman, Saeed Saad, Ng, Tzi Bun
Journal:
PROTEIN AND PEPTIDE LETTERS BENTHAM SCIENCE PUBL LTD
Volume:
18
Research Area:
Biochemistry \& Molecular Biology
ISSN
ISI:000286221400009
Keywords :
Apoptosis, hepatoma, purification, trypsin inhibitor, Vicia faba
Abstract:
A new 15-kDa Bowman-Birk type trypsin inhibitor (termed VFTI-G1) was isolated from the seeds of Faba bean (Vicia faba cv. Giza 843) using cation exchange chromatography on an SP-Sepharose column, anion exchange chromatography on Q-Sepharose and Mono Q columns, and finally size exclusion chromatography on a Superdex 75 column. VFTI-G1 manifested significant antiproteolytic activity against trypsin (5761 BAEE units/mg, K(i) 20.4 x 10(-9) M), but only a slight chymotrypsin inhibitory activity (< 10 BTEE units/mg). The suitable environment to sustain its trypsin inhibitory activity was at temperatures below 60 degrees C and at pH 7. Its trypsin inhibitory activity was inhibited by the reducing agent dithiothreitol in a dose-dependent manner, indicating the significance of intact disulfide bonds to the trypsin inhibitory activity. It inhibited HIV-1 reverse transcriptase (RT) activity with an IC(50) of about 0.76 mu M. Furthermore, VFTI-G1 showed specific antiproliferative activity toward HepG2 hepatoma cells by inducing chromatin condensation and cell apoptosis. The HIV-1 RT inhibitory activity of VFTI-G1 and its specific antiproliferative activity toward Hep G2 cells may find medical applications.
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