DNA Image Cytometry of the Liver of Chronic Hepatitis C Virus Infected Patients and Hepatocellular Carcinoma in Sharkia Governorate in Egypt

In the present investigation forty three (11 HCC, 12 cirrhosis and 20 chronic hepatitis) cases were chosen from the paraffin blocks and unstained slides of fine needle aspirates biopsies which were preserved in the archive of the Early Cancer Detection Unit (ECDU) belonging to the Faculty of medicine, Zagazig University, Egypt. All the cases were previously diagnosed and proved by PCR to have HCV. 50 mu m thick paraffin sections and fine needle aspirates smears were prepared for evaluating DNA ploidy and S- phase fraction (SPF) by DNA image analysis. The results indicated that, the hepatocyts of chronic hepatitis C cases have a regular multiplicity of normal diploid DNA (Euploid polyploidization). All of the studied HCCs cases and only one cirrhotic case revealed aneuploidy. It was concluded that, patients infected with chronic HCV are predominantly affected by HCC and the DNA image analysis techniques can be considered as early predictors of cellular abnormality and different malignant criteria, which can lead to early and well diagnosis as well as rapid manipulation of the patients. {[}Samia, M. Sanad, Amal, M. Mangoud, Amr A. Shalaby and Mahmoud S. Abd El-Wahed zar. DNA Image Cytometry of the Liver of Chronic Hepatitis C Virus Infected Patients and Hepatocellular Carcinoma in Sharkia Governorate in Egypt. Life Science Journal 2011;8(4):987-995]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 126.