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RP-HPLC/Pre-Column Derivatization for Analysis of Omeprazole, Tinidazole, Doxycycline and Clarithromycin
Faculty
Pharmacy
Year:
2013
Type of Publication:
Article
Pages:
566-576
Authors:
Mostafa, Samia, Darwish, Khaled M, Salama, Ismail, El-Sadek, Mohamed
DOI:
10.1093/chromsci/bms167
Journal:
JOURNAL OF CHROMATOGRAPHIC SCIENCE OXFORD UNIV PRESS INC
Volume:
51
Research Area:
Biochemistry \& Molecular Biology; Chemistry
ISSN
ISI:000322831200012
Keywords :
RP-HPLC/Pre-Column Derivatization , Analysis , Omeprazole, Tinidazole, Doxycycline
Abstract:
A validated, reliable and accurate reversed-phase high performance liquid chromatographic method using pre-column derivatization was adopted for the simultaneous determination of two ternary mixtures containing omeprazole, tinidazole and doxycycline hyclate or clarithromycin. Separation was achieved on a C18 column, through a gradient elution system using acetonitrile-methanol-water adjusted to pH = 6.60. Drugs were detected at 277 nm over concentration ranges of 1-112, 5-125, 2.5-550 and 2.5-100 mu g/mL for omeprazole, tinidazole, doxycycline hyclate and clarithromycin, respectively. This is the first method that has isolated and identified clarithromycin derivative by infrared and mass spectroscopy. This method is the first study for the simultaneous determination of omeprazole, tinidazole, doxycycline hyclate and clarithromycin in combined mixtures and pharmaceutical formulations.
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