Type-A Nucleophosmin (Npm1) Gene Mutation As A Prognostic Marker In Myelodysplastic Syndrome Patients With Normal Karyotypes

Fresh blood samples were aspirated from MDS patients before their treatment began, and from volunteers. 6 mL venous blood samples were withdrawn, delivered into two tubes:• 1 mL was delivered into EDTA vacutainer tubes, and then gently inverted then CBC was performed• 5 mL were delivered into sterile plain vacutainer tubes with stopper, left to clot at 37C for 10 minutes, then centrifuged at 3000 rpm for 10 minutes, then the resulting serum was used for liver and kidney functions, LDH and Ferritin analysisIn addition, 1.5 mL bone marrow samples were aspirated from MDS patients on referral for diagnosis, and were divided into:• 1.0 mL was delivered to Lithium Heparin containing polypropylene tube with stopper, for conventional cytogenetic study• 0.5 mL was used for preparation of bone marrow smears, stained with Leishman stainAll samples were processed within 4 hours from collection.