Chemical Composition of the Essential Oils of Variegated Pink-Fleshed Lemon (Citrus x limon L. Burm. f.) and their Anti-Inflammatory and Antimicrobial Activities

The volatile secondary metabolites of essential oils from fruit peel and leaves of variegated pink-fleshed lemon (Citrus x limon) were investigated using GLC and GLC-MS (gas-liquid chromatography-mass spectroscopy). Altogether 141 compounds were identified and quantified, accounting for 99.59\% and 96.33\% of the total hydrodistilled peel and leaf oil, respectively. Limonene occurred in higher amounts in fruit peel (52.73\%) than in leaf oil (29.13\%). Neral (12.72\%), neryl acetate (8.53\%), rho-menth-1-en-7-al (4.63\%), beta-pinene (6.35\%), and nerol (4.42\%) were the most abundant constituents in leaf oil, whereas gamma-terpinene (9.88\%), beta-pinene (7.67\%), geranial (4.44\%), and neral (3.64\%) dominated in the fruit peel oil. The antioxidant, anti-inflammatory, antitrypanosomal, and antimicrobial activities of the fruit peel essential oil were evaluated. The oil had a low antioxidant activity with an IC50 value of (26.66 +/- 2.07) mg/ml as compared to the efficient antioxidant ascorbic acid {[}IC50(16.32 +/- 0.16) mu g/ml]. The oil moderately inhibited soybean 5-lipoxygenase (5-LOX) with an IC50 value of (32.05 +/- 3.91),mu g/ml and had moderate antitrypanosomal activity {[}IC50 (60.90 +/- 0.91) mu g/ml]. In addition, moderate antimicrobial activities were detected against Gram-positive bacteria (Bacillus subtilis, Staphylococcus capitis, Micrococcus luteus), one Gram-negative bacterium (Pseudomonas fluorescens), and yeasts (Saccharomyces cerevisiae, Candida parapsilosis).