Multi-polymerase chain reaction diagnostic method for detection of toxins excretion third microbe Pseudomonas Ierginosa

Pseudomonas aeruginosa is an opportunistic Gram-negative bacillus that causes a variety of clinically important infections in immunocompromised hosts and in critically ill patients, commonly affecting patients with cystic fibrosis, severe burns, neutropenia and the mechanically ventilated patients. It has emerged as one of the most problematic Gram-negative bacteria in hospital settings, causing 15 to 20% of cases of hospital-acquired pneumonia. Infection is associated with mortality rates as high as 70% overall, and 90% in mechanically-ventilated patients.