Diabetic nephropathy (DN) is one of most serious long-term complications in diabetic patients. DN has been viewed as an ascending path from normoalbuminuria to End Stage renal disease (ESRD) through intermediate stages marked by microalbuminuria and overt proteinuria causing considerable morbidity and mortality.The aim of this work is to find out the relation between the duration of DM and the level of TGF-1 in blood. We evaluated the possibility of using blood biomarker as TGF-1 level as a diagnostic marker for onset of diabetic nephropathy and tested if its level increased with time.In the present study, plasma concentrations of TGF-1 and their correlations with duration of diabetes and microalbuminuria were evaluated in 75 individuals, classified into three group including 15 healthy volunteers as control (group I), 30 type 2 diabetic patients without complications (group II) and 30 diabetic patients with early DN ((group III).