PCR based assay of interleukin-13 receptor gene polymorphism and its blood level as biochemical marker in atopic patients

The bioactivity of IL 13 a Th2 cytokine which is an important regulator of inflammatory immune responses is mediated through a receptor complex consisting of IL-13Rα1 and IL-4Rα chain which is very important in IL-13 and IL-4 dependent signaling.IL-13Rα2 binds IL-13 with high affinity which is thought to act primarily as a ‘‘decoy’’ receptor, sequestering IL-13 from the IL-13Rα1/IL-4Rα complex, and thus inhibiting its function.Our aim is to clarify the role of these receptors in the diagnosis and follow-up of atopic patients.Methods:We genotyped IL-13Rα1 polymorphism at position +1398A/G searching for a causal genetic diversity and measured serum level of IL-13Rα2 in 105 atopic patients suffering from atopic asthma, atopic dermatitis and atopic rhinitis (35 each) and compared the results to 35 non atopic control subjects.Total immunoglobulin (Ig) E and serum IL-13Rα2 were measured using enzyme-linked immunosorbent assay,Blunt abdominal trauma (BAT) is a leading cause of morbidity and mortality among all age groups. Identification of serious intra-abdominal pathology is often challenging. Many injuries may not manifest during the initial assessment and treatment period. Mechanisms of injury often result in other associated injuries that may divert the physician’s attention from potentially life-threatening intra-abdominal pathology.