A study on the role of Pituitary gonadotropins in experimentally-induced osteoporosis in female rats

In a trial to identify the role of pituitary gonadotrophins in post menopausal osteoporosis; the present work had been carried on a total number of 70 adult female albino rats.The animals were divided into 7 groups:Group (1) (control group): Was consisted of 10 sham operated female albino rats. Group (2) (OV): Was consisted of 10 ovariectomized albino rats. Group (3) (OV + E): Was consisted of 10 ovariectomized albino rats treated with estradiol. The rats were injected subcutaneously with estrogen for 6 weeks . Group (4) (OV + FSH): Was consisted of 10 ovariectomized albino rats treated with FSH injection. The rats were injected subcutaneously with FSH for 6 weeks .Group (5) (OV + LH): Was consisted of 10 ovariectomized albino rats treated with LUPRON. Group (6) (OV+ E+ FSH): Was consisted of 10 ovariectomized albino rats treated with both estrogen and FSH. The rats were injected subcutaneously with estradiol and FSH. Group (7) (OV+ E+ LH): Was consisted of 10 ovariectomized albino rats receiving both estradiol and LH. The rats were injected subcutaneously daily with and with lupron .there was a significant negative correlation between serum gonadotrophins levels and bone parameters in all groups and there was a significant positive correlation between serum gonadotrophins levels and serum alkaline phosphatase in all groups. Moreover, was a significant positive correlation between serum gonadotrophins levels and serum TNF-α in all groups except ovariectomized treated with estradiol +LH group.However, there was no significant correlation between serum estradiol and bone parameter, serum alkaline phosphatase or serum TNF-α in all studied groups. In conclusion from the previous data it was found that pituitary gonadotrophins can decrease bone mineral density regardless the level of estrogen in ovariectomized rats.