Fluorescence probe enhanced spectrofluorimetric method for the determination of sparfloxacin in tablets and biological fluids

Yttrium-sensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of sparfloxacin. The method is based on the strong fluorescence of sparfloxacin after adding the fluorescence probe yttrium in buff er solution (pH = 8), and various factors influencing fluorescence were investigated. Under optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of sparfloxacin over the range 8 x 10(-7) to 1.4 x 10(-5) mol L(-1) with a correlation coefficient of 0.9997. The detection limit (S/N = 3) was determined as 9.01 x 10(-8) mol L(-1). The mechanism of the sensitizing effect of probe was discussed. This method has been successfully applied for the determination of sparfloxacin in pharmaceuticals, human urine and serum samples; the result obtained was satisfactory. Copyright (c) 2010 John Wiley \& Sons, Ltd.