Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center
Faculty
Veterinary Medicine
Year:
2011
Type of Publication:
Article
Pages:
428-432
Authors:
Hochi, Shinichi, Abdalla, Hany, Hara, Hiromasa, Hirabayashi, Masumi, Morita, Hiroshi, Kuwayama, Masashige
DOI:
10.1262/jrd.10-167N
Journal:
JOURNAL OF REPRODUCTION AND DEVELOPMENT JAPANESE SOC ANIMAL REPRODUCTION
Volume:
57
Research Area:
Agriculture; Reproductive Biology
ISSN
ISI:000292435600017
Keywords :
Bovine Intracytoplasmic sperm injection (ICSI), Freeze-drying, Microtubule-organizing center (MTOC), Sperm-aster
Abstract:
This study was designed to investigate whether freeze-dried (FD) bull spermatozoa maintained the function of the microtubule-organizing center (MTOC) after rehydration and intracytoplasmic sperm injection (ICSI). In a preliminary attempt, the cleavage and blastocyst formation rates in FD-ICSI zygotes (36 and 1\%, respectively) were found to be considerably lower than those in control ICSI zygotes (67 and 21\%, respectively) or in IVF zygotes (78 and 43\%, respectively). An alkaline comet assay indicated that the DNA fragmentation index (length of comet tail x \% DNA liberated) was not significantly different between fresh and FD spermatozoa. In the main experiment, formation of sperm-asters in the FD-ICSI oocytes 7 h postinsemination occurred at a similar rate when compared with the control ICSI oocytes (41 vs. 49\%). Among the oocytes exhibiting sperm aster formation, the extent of microtubule network assembly was comparable between the FD-ICSI and control ICSI groups. However, the MTOC of the ICSI oocytes was not as functional as that of IVF oocytes in terms of the aster formation rate (97\%) and the fluorescent intensity of the microtubule network (2.0 folds). These results suggest that the freeze-drying process per se had no adverse effect on maintaining the MTOC function in bull spermatozoa.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف