Immunodiagnosis of Acute Toxoplasmosis in Sera and Urine of Experimentally Infected Mice

Toxoplasmosis is one of the more common parasitic zoonoses world wide. Infection occurs in human through ingestion of tissue cysts in raw or inadequately cooked meat of infected animals or via ingestion of food containing oocysts. Infection with T. gondii is usually asymptomatic in immunocompetent individuals. However, in immunocompromised host, tissue destruction of specific organs or dissimination of the disease may occur. Also, during pregnancy toxoplasmosis may cause abortion, still birth or great damage to the baby may occur so accurate diagnosis is essential for early detection of infection and avoidance of complications.This work aimed to evaluate the role of ELISA and RLA tests in diagnosis of acute toxoplasmosis through the detection of circulating antigens in sera and urine of experimentally infected mice by using 60 Swiss Albino mice classified into:1- Control group: non infected 10 mice2- Infected group: 50 mice infected with virulent RH strain of T. gondii.Infected mice were scarified at 3rd day post infection, sera were separated and urine was collected from the infected and control group then stored at -20 till used.ELISA and RLA tests were done for detection of circulating Toxoplasma antigens in sera and urine samples of infected mice.The following results were analyzed and tabulated;A- ELISA results showed:1- High significant increase in serum antigens concentration compared to control group. The percentage positivity was (94%).