P and G Genotyping ()Frota Virus Strains, Detection Of Vp4and Vp7 Gene Frequency In Diarrheal Stool

Detection and typing of group A rotavirus strains are clone by sensitive tests, which arc needed lor giving more knowledge about infection with rotavirus, The knowledge of relative frequency and distribution of rotavirus serotypes/genotypes can help in the analysis of regional differences and variation over the years in population samples.Our approach in this study is G and P genotyping of rotavirus Il1 diarrheal stool by reverse transcription PCR via amplification of entire VP7 and VP4 genes. Then determining their frequency and relation to severity of diarrhea allowing rapid epidemiological survey of rotavirus. We present the results in comparison with conventional methods for detection of rotavirus as latex agglutination and ELISA.This study was carried out on 30 infants with acute gastroenteritis, their age ranged from 2-12 months and 10 healthy infant of the same age and sex group served as control. All infants are subjected to medicalexamination and medical history from their parents. Stool samples of all infants were examined macroscopically and microscopically and ”cultured on special media to exclude associatedbacterial infection then specific and sensitive tests for detection of rotavirus as latex agglutination and ELISA technique were done. Rt-PCR was done for all samples for detection and genotyping of rotavirus.