Bacteriological and partial study on clinical isolates microbe Acrkih the coli type and 157

The recognition of Escherechia coli 0157 have emerged asimportant human enteric pathogens. Strains which express thelipopolysaccharide (LPS) 0-antigen 157 (0157 strain) are commonlyassociated with sever clinical manifestations, including bloody diarrhea,hemorrhagic colitis and hemolytic uremic syndrome. Diseases caused byE. coli 0157 occur sporadically, in small clusters and outbreaks, and maybe transmitted in a variety of ways, including food, water, person-to-person and animal-to-person contact.With the emergence of E. coli 0157, diagnostic tests becameavailable to facilitate detection of this organism including: Selectiveagars, detection of genes characteristic of E. coli 0157 strains and theImmunological assay for the detection of E. coli 0157 LPS antigen.This study aimed to isolate E. coli 0157 pathogenic strains fromclinical samples, determine the antimicrobial susceptibility patterns andshed some light on PCR for detection of this organism in comparisonwith the conventional methods. This work was conducted on 273 patients attending ZagazigUniversity Hospitals outpatients clinics presented with non-bloodydiarrhea, bloody diarrhea and acute renal failure. Their age rangesbetween 9 months to 67 years. All investigations were conducted in theMicrobiology and Immunology department Faculty of Medicine ZagazigUniversity. Stool samples were collected from all patients and subjected to:I. Direct smear 2. Cultivation on Nutrient, blood, MacConkey’s and sMac agars.