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Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa
Faculty
Veterinary Medicine
Year:
2011
Type of Publication:
Article
Pages:
557-563
Authors:
Hochi, Shinichi, Abdalla, Hany, Hara, Hiromasa, Hirabayashi, Masumi
Journal:
JOURNAL OF REPRODUCTION AND DEVELOPMENT SOCIETY REPRODUCTION \& DEVELOPMENT-SRD
Volume:
57
Research Area:
Agriculture; Reproductive Biology
ISSN
ISI:000296597100001
Keywords :
Freeze-drying, Lyophilization, ICSI, Spermatozoa
Abstract:
Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose their motility, which is an essential requirement to complete physiological fertilization, a relatively difficult microinsemination technique must be applied to rehydrated spermatozoa. Theoretically, it has been supposed that freeze-dried spermatozoa could maintain their functions and abilities to interact with the oocyte cytoplasm after prolonged storage at refrigerator temperature. However, sufficient yield of transferable blastocysts and production of live offspring derived from freeze-dried sperm samples are still subjects to be challenged and overcome in large domestic species.
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