The hepatitis G virus (HGVlGBV-C) is recently discovered viralagent cloned from the serum of individuals with hepatitis. Like Hey, HOYappears to result commonly in chronic infection. The relationship of HOYinfection with liver disease and possible interaction between HGYandknown hepatitis viruses have also been estimated. However, so far no clearlink has been established between HOV and chronic liver disease.We aimed to study HGY infection in patients with chronic renalfailure under maintenance hemodialysis, intermittent peritonealdialysis.personal who deal with patients (doctors ,nurses worker ) innephrology unit Zagazig University hospital using different laboratorydiagnostic test.The present work included 40 patients on maintenance hemodialysis[29 males and 11 females with a mean age of(43.6±14.7) years and rangedfrom 20 to 72 years] and 20 patients on intermittent peritoneal dialysis [6males and 14 females with a mean age of(52.9±1O.7) years and rangedfrom 16 to 64 years]. And 10 persons who deal with patients on nephrologyunit [5males and 5 females with a mean age of(33.4±11.l7) years andranged from 24to 56 years]. In addition, 10apparently healthy individuals[(8 males and 2 females with a mean age of(33±9.7) years and ranged from20 to 52 years] with no previous history ofblood transfusion as a controlgroup.All patients and controls were subjected to complete history takingand some routine investigations as well as serological assays for detectionof anti-HOV-E2, HBsAg, Hlv-Ab as well as HCV-IgG using ELISAtechniques. HOV-RNA and HeV-RNA were assayed by RT·PCR.