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Production of Transgenic Bovine Cloned Embryos Using Piggybac Transposition
Faculty
Veterinary Medicine
Year:
2011
Type of Publication:
Article
Pages:
1453-1457
Authors:
Saadeldin, Islam M, Jang, Goo, Lee, Byeong Chun, Kim, Su Jin, Choi, Woo Jae, Lee, Song Jeon, Lee, Won Wu, Kim, Bong Han, Han, Hyo Jun, Bang, Du Hee
Journal:
JOURNAL OF VETERINARY MEDICAL SCIENCE JAPAN SOC VET SCI
Volume:
73
Research Area:
Veterinary Sciences
ISSN
ISI:000298871500009
Keywords :
bovine embryos, GFP, piggybac, RFP, SCNT
Abstract:
Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RH) expression was regulated by doxycycline treatment in donor fibroblasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.
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