A study on the effect of colony stimulating activity on normal and on some bone marrow hypoplastic conditions

Colony stimulating factors are the only agents that can directly stimulate stem cell progenitors unlike other growth factors that act indirectly. They stimulate stem cell proliferation, differentiation and also improve the function of mature neutrophils. In our work, we examined 54 animal divided into 5 groups.1st, 2nd groups included 6 animals each. Their marrow speer were cultured both with and without adding P.E. ( group II and I ) respect- ively. It was observed that addition of P.E. increases the colony growth ( P 0.0005 ). Other cultures have been done after whole body irradiation of animals of group IV ( 18 animals ), the cultures were set with and without .adding P.E. , P.E. significantly increases ( P < 0.0005 ) the number of colonies grown when compared with control group to which No. P.E. was added. Animals of the 3 rd & 5th groups are irradiated to induce bone marrow depression. The animals of group III ( 18 animals ) are left for spontaneous recovery. Animals of group V are injected intraper- itionealy with the extract and follow up of their recovery was assessed during the following 3 weeks. Comparison between group III and group V revealed rapid and better recovery of group V.This is an assesse- ment of the in vivo function of the extract and it is very similar to its in vit ro action.