Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Extractive Spectrophotometric Determination of Anti-Inflammatory Drug Nimesulide in Pharmaceutical Formulations and Human Plasma
Faculty
Science
Year:
2011
Type of Publication:
Article
Pages:
429-436
Authors:
Saber, Amr Lotfy, El-Sayed, Gamal Owes
Journal:
JOURNAL OF FOOD AND DRUG ANALYSIS BUREAU FOOD DRUG ANALYSIS
Volume:
19
Research Area:
Food Science \& Technology; Pharmacology \& Pharmacy
ISSN
ISI:000298774100006
Keywords :
Nimesulide, extractive spectrophotometry, pharmaceutical, human plasma
Abstract:
The goal of the present work is to develop simple, rapid, and sensitive extractive spectrophotometric methods for the assay of nimesulide in pharmaceutical formulations and human plasma. The methods depend on the formation of colored ion-pair complexes between nimesulide and Five different reagents, bromocresol green (BCG), bromocresol purple (BCP), bromothymol blue (BTB), brilliant blue G (BBG) and methyl orange (MO) in Britton-Robinson buffer solutions of pH 2.0 - 3.5. The colored products were extracted in chloroform and absorbance measured at 412, 410, 407, 502 and 482 nm, respectively. The analytical parameters and their effects on the reported systems were investigated. The extracts are intensely colored and very stable at room temperature. The calibration graphs were linear over the concentration range of 2 - 18 mu g/mL for BCG, BTB and BBG, 2 - 16 mu g/mL for BCP and 2 - 14 mu g/mL for MO. The stoichiometry of the reaction was found to be 1 : 1 in all cases and the conditional stability constant (K-f) of the complexes were calculated. The proposed methods were successfully extended to pharmaceutical preparations in tablet form. Excipients used as additives in commercial formulations did not interfere with the analysis. The proposed methods can be recommended for quality control and routine analysis where time, cost effectiveness and high specificity of the analytical technique are of great importance.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف