Apolipoprotein E Genotypes As A Risk Factor For Myocardial Infarction

Apolipoprotein (apo E) plays a central role in the metabolism of cholesterol and triglycerides. It is one of the major protein constituents of chylomicrons, VLDL, their remnant particles and HDL. On these particles apo E serves as a ligand for their uptake by lipoprotein receptors.Apolipoprotein E is a polymorphic protein with three major isoforms, apo E2, apo E3 and apo E4. The biosynthesis of these isoforms is under the control of three independent codominant alleles, apo E2, apo E3 and apo E4, at a single apo E gene locus on chromosome 19.The study of the genetic variability at the apo E gene locus has aroused considerable interest in recent years as this genetic variation seems to be a major determinant of plasma lipid concentration and of the interindividual variations in susceptibility to coronary artery disease.The aim of this study was to clarify the role of apo E polymorphism as risk factor for development of CHD and to investigate the relationship between apo E polymorphism and several clinical and biochemical markers of CHD.This study was conducted on 26 patients, with past history of myocardial infarction and Twelve healthy age and sex matched controls.Studied subjects were subjected to the following laboratory investigations:.1- Thourough history taking, clinical examination.2- Serum total cholesterol, triglycerides, HDL-C and LDL-C levels.3- Serum glucose and uric acid.Apolipoprotien E genotyping by LightCycler Instrument.4