Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants

Faculty	Pharmacy	Year:	2012
Type of Publication:	Article	Pages:	604-606
Authors:	Soliman, Sameh S. M, Raizada, Manish N, Tessaro, Michael J	DOI:	10.1128/AEM.05261-11
Journal:	APPLIED AND ENVIRONMENTAL MICROBIOLOGY AMER SOC MICROBIOLOGY	Volume:	78
Research Area:	Biotechnology \& Applied Microbiology; Microbiology	ISSN	ISI:000299018800040
Keywords :	Bacterial Whole-Cell Biosensor , Glutamine with Applications
Abstract:	A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gin) auxotroph with a constitutive lux reporter gene. Measurements of Gin in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gin from whole plant organs.
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